Review





Similar Products

matchmaker gold yeast two hybrid system  (TaKaRa)
99
TaKaRa matchmaker gold yeast two hybrid system
CsRNF interacts with CsHSF24 in vitro and in vivo. (A) BiFC assay demonstrating the interaction between CsHSF24 and CsRNF in N. benthamiana leaf cells. YFP fluorescence indicates successful interaction. Scale bar = 50 μm. EV = empty vector control; CsHSF24‐BC/CsRNF‐BC = background controls for CsHSF24 and CsRNF, respectively. Interaction test: Coexpression of YFPN‐CsHSF24 and YFPC‐CsRNF. (B) <t>Yeast</t> <t>two‐hybrid</t> assay confirming the interaction between CsRNF and CsHSF24. Serial dilutions (10 −1 to 10 −5 ) of yeast cultures were spotted onto selective media. (C) Schematic diagrams of the reporter and effector constructs used in dual‐luciferase assays. (D, E) Dual‐luciferase assay showing that CsRNF represses CsHSF24‐mediated activation of the CsF3′5′H2 promoter. (F) A pull‐down assay of the interaction between CsRNF and CsHSF24. Recombinant GST‐CsHSF24 was incubated with immobilized His‐CsRNF and detected for immunoblot analysis using an anti‐His antibody (upper panel) or an anti‐GST antibody (bottom panel). The arrow represents the target protein. (G) The expression level of CsRNF in leaves of one wild tea plant (“Siqiu” (“SQ”)) and three tea cultivars (“Wuniuzao” (“WNZ”), “Longjing43” (“LJ43”), “Zhongcha108” (“ZC108”)). Error bars: ±SD. Statistical significance was determined using Tukey's multiple range test ( P < 0.05).
Matchmaker Gold Yeast Two Hybrid System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two/Matchmaker+Gold+Yeast+Two-Hybrid+System/pmc13651704-305-14-19
Average 99 stars, based on 1 article reviews
matchmaker gold yeast two hybrid system - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
two hybrid system 3  (TaKaRa)
99
TaKaRa two hybrid system 3
CsRNF interacts with CsHSF24 in vitro and in vivo. (A) BiFC assay demonstrating the interaction between CsHSF24 and CsRNF in N. benthamiana leaf cells. YFP fluorescence indicates successful interaction. Scale bar = 50 μm. EV = empty vector control; CsHSF24‐BC/CsRNF‐BC = background controls for CsHSF24 and CsRNF, respectively. Interaction test: Coexpression of YFPN‐CsHSF24 and YFPC‐CsRNF. (B) <t>Yeast</t> <t>two‐hybrid</t> assay confirming the interaction between CsRNF and CsHSF24. Serial dilutions (10 −1 to 10 −5 ) of yeast cultures were spotted onto selective media. (C) Schematic diagrams of the reporter and effector constructs used in dual‐luciferase assays. (D, E) Dual‐luciferase assay showing that CsRNF represses CsHSF24‐mediated activation of the CsF3′5′H2 promoter. (F) A pull‐down assay of the interaction between CsRNF and CsHSF24. Recombinant GST‐CsHSF24 was incubated with immobilized His‐CsRNF and detected for immunoblot analysis using an anti‐His antibody (upper panel) or an anti‐GST antibody (bottom panel). The arrow represents the target protein. (G) The expression level of CsRNF in leaves of one wild tea plant (“Siqiu” (“SQ”)) and three tea cultivars (“Wuniuzao” (“WNZ”), “Longjing43” (“LJ43”), “Zhongcha108” (“ZC108”)). Error bars: ±SD. Statistical significance was determined using Tukey's multiple range test ( P < 0.05).
Two Hybrid System 3, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two/Matchmaker+Gold+Yeast+Two-Hybrid+System/pm42827260-428-8-11
Average 99 stars, based on 1 article reviews
two hybrid system 3 - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
two hybrid system  (TaKaRa)
99
TaKaRa two hybrid system
CsRNF interacts with CsHSF24 in vitro and in vivo. (A) BiFC assay demonstrating the interaction between CsHSF24 and CsRNF in N. benthamiana leaf cells. YFP fluorescence indicates successful interaction. Scale bar = 50 μm. EV = empty vector control; CsHSF24‐BC/CsRNF‐BC = background controls for CsHSF24 and CsRNF, respectively. Interaction test: Coexpression of YFPN‐CsHSF24 and YFPC‐CsRNF. (B) <t>Yeast</t> <t>two‐hybrid</t> assay confirming the interaction between CsRNF and CsHSF24. Serial dilutions (10 −1 to 10 −5 ) of yeast cultures were spotted onto selective media. (C) Schematic diagrams of the reporter and effector constructs used in dual‐luciferase assays. (D, E) Dual‐luciferase assay showing that CsRNF represses CsHSF24‐mediated activation of the CsF3′5′H2 promoter. (F) A pull‐down assay of the interaction between CsRNF and CsHSF24. Recombinant GST‐CsHSF24 was incubated with immobilized His‐CsRNF and detected for immunoblot analysis using an anti‐His antibody (upper panel) or an anti‐GST antibody (bottom panel). The arrow represents the target protein. (G) The expression level of CsRNF in leaves of one wild tea plant (“Siqiu” (“SQ”)) and three tea cultivars (“Wuniuzao” (“WNZ”), “Longjing43” (“LJ43”), “Zhongcha108” (“ZC108”)). Error bars: ±SD. Statistical significance was determined using Tukey's multiple range test ( P < 0.05).
Two Hybrid System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two/Matchmaker+Gold+Yeast+Two-Hybrid+System/pm42809140-78-7-9
Average 99 stars, based on 1 article reviews
two hybrid system - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
photon microscope nikon a1r mp multiphoton upright microscope  (Nikon)
96
Nikon photon microscope nikon a1r mp multiphoton upright microscope
CsRNF interacts with CsHSF24 in vitro and in vivo. (A) BiFC assay demonstrating the interaction between CsHSF24 and CsRNF in N. benthamiana leaf cells. YFP fluorescence indicates successful interaction. Scale bar = 50 μm. EV = empty vector control; CsHSF24‐BC/CsRNF‐BC = background controls for CsHSF24 and CsRNF, respectively. Interaction test: Coexpression of YFPN‐CsHSF24 and YFPC‐CsRNF. (B) <t>Yeast</t> <t>two‐hybrid</t> assay confirming the interaction between CsRNF and CsHSF24. Serial dilutions (10 −1 to 10 −5 ) of yeast cultures were spotted onto selective media. (C) Schematic diagrams of the reporter and effector constructs used in dual‐luciferase assays. (D, E) Dual‐luciferase assay showing that CsRNF represses CsHSF24‐mediated activation of the CsF3′5′H2 promoter. (F) A pull‐down assay of the interaction between CsRNF and CsHSF24. Recombinant GST‐CsHSF24 was incubated with immobilized His‐CsRNF and detected for immunoblot analysis using an anti‐His antibody (upper panel) or an anti‐GST antibody (bottom panel). The arrow represents the target protein. (G) The expression level of CsRNF in leaves of one wild tea plant (“Siqiu” (“SQ”)) and three tea cultivars (“Wuniuzao” (“WNZ”), “Longjing43” (“LJ43”), “Zhongcha108” (“ZC108”)). Error bars: ±SD. Statistical significance was determined using Tukey's multiple range test ( P < 0.05).
Photon Microscope Nikon A1r Mp Multiphoton Upright Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two/A1+MP%2B+%2F+A1R+MP%2B/pm42790565-117-10-12
Average 96 stars, based on 1 article reviews
photon microscope nikon a1r mp multiphoton upright microscope - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
647 jo urn al pr e p roo f matchmaker gold yeast two hybrid system  (TaKaRa)
99
TaKaRa 647 jo urn al pr e p roo f matchmaker gold yeast two hybrid system
CsRNF interacts with CsHSF24 in vitro and in vivo. (A) BiFC assay demonstrating the interaction between CsHSF24 and CsRNF in N. benthamiana leaf cells. YFP fluorescence indicates successful interaction. Scale bar = 50 μm. EV = empty vector control; CsHSF24‐BC/CsRNF‐BC = background controls for CsHSF24 and CsRNF, respectively. Interaction test: Coexpression of YFPN‐CsHSF24 and YFPC‐CsRNF. (B) <t>Yeast</t> <t>two‐hybrid</t> assay confirming the interaction between CsRNF and CsHSF24. Serial dilutions (10 −1 to 10 −5 ) of yeast cultures were spotted onto selective media. (C) Schematic diagrams of the reporter and effector constructs used in dual‐luciferase assays. (D, E) Dual‐luciferase assay showing that CsRNF represses CsHSF24‐mediated activation of the CsF3′5′H2 promoter. (F) A pull‐down assay of the interaction between CsRNF and CsHSF24. Recombinant GST‐CsHSF24 was incubated with immobilized His‐CsRNF and detected for immunoblot analysis using an anti‐His antibody (upper panel) or an anti‐GST antibody (bottom panel). The arrow represents the target protein. (G) The expression level of CsRNF in leaves of one wild tea plant (“Siqiu” (“SQ”)) and three tea cultivars (“Wuniuzao” (“WNZ”), “Longjing43” (“LJ43”), “Zhongcha108” (“ZC108”)). Error bars: ±SD. Statistical significance was determined using Tukey's multiple range test ( P < 0.05).
647 Jo Urn Al Pr E P Roo F Matchmaker Gold Yeast Two Hybrid System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two/Matchmaker+Gold+Yeast+Two-Hybrid+System/pm42798183-332-17-30
Average 99 stars, based on 1 article reviews
647 jo urn al pr e p roo f matchmaker gold yeast two hybrid system - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
matchmaker yeast two hybrid system  (TaKaRa)
99
TaKaRa matchmaker yeast two hybrid system
CsRNF interacts with CsHSF24 in vitro and in vivo. (A) BiFC assay demonstrating the interaction between CsHSF24 and CsRNF in N. benthamiana leaf cells. YFP fluorescence indicates successful interaction. Scale bar = 50 μm. EV = empty vector control; CsHSF24‐BC/CsRNF‐BC = background controls for CsHSF24 and CsRNF, respectively. Interaction test: Coexpression of YFPN‐CsHSF24 and YFPC‐CsRNF. (B) <t>Yeast</t> <t>two‐hybrid</t> assay confirming the interaction between CsRNF and CsHSF24. Serial dilutions (10 −1 to 10 −5 ) of yeast cultures were spotted onto selective media. (C) Schematic diagrams of the reporter and effector constructs used in dual‐luciferase assays. (D, E) Dual‐luciferase assay showing that CsRNF represses CsHSF24‐mediated activation of the CsF3′5′H2 promoter. (F) A pull‐down assay of the interaction between CsRNF and CsHSF24. Recombinant GST‐CsHSF24 was incubated with immobilized His‐CsRNF and detected for immunoblot analysis using an anti‐His antibody (upper panel) or an anti‐GST antibody (bottom panel). The arrow represents the target protein. (G) The expression level of CsRNF in leaves of one wild tea plant (“Siqiu” (“SQ”)) and three tea cultivars (“Wuniuzao” (“WNZ”), “Longjing43” (“LJ43”), “Zhongcha108” (“ZC108”)). Error bars: ±SD. Statistical significance was determined using Tukey's multiple range test ( P < 0.05).
Matchmaker Yeast Two Hybrid System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two/Matchmaker+Gold+Yeast+Two-Hybrid+System/pm42785402-94-29-33
Average 99 stars, based on 1 article reviews
matchmaker yeast two hybrid system - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
activity optimized sequences  (Addgene inc)
93
Addgene inc activity optimized sequences
CsRNF interacts with CsHSF24 in vitro and in vivo. (A) BiFC assay demonstrating the interaction between CsHSF24 and CsRNF in N. benthamiana leaf cells. YFP fluorescence indicates successful interaction. Scale bar = 50 μm. EV = empty vector control; CsHSF24‐BC/CsRNF‐BC = background controls for CsHSF24 and CsRNF, respectively. Interaction test: Coexpression of YFPN‐CsHSF24 and YFPC‐CsRNF. (B) <t>Yeast</t> <t>two‐hybrid</t> assay confirming the interaction between CsRNF and CsHSF24. Serial dilutions (10 −1 to 10 −5 ) of yeast cultures were spotted onto selective media. (C) Schematic diagrams of the reporter and effector constructs used in dual‐luciferase assays. (D, E) Dual‐luciferase assay showing that CsRNF represses CsHSF24‐mediated activation of the CsF3′5′H2 promoter. (F) A pull‐down assay of the interaction between CsRNF and CsHSF24. Recombinant GST‐CsHSF24 was incubated with immobilized His‐CsRNF and detected for immunoblot analysis using an anti‐His antibody (upper panel) or an anti‐GST antibody (bottom panel). The arrow represents the target protein. (G) The expression level of CsRNF in leaves of one wild tea plant (“Siqiu” (“SQ”)) and three tea cultivars (“Wuniuzao” (“WNZ”), “Longjing43” (“LJ43”), “Zhongcha108” (“ZC108”)). Error bars: ±SD. Statistical significance was determined using Tukey's multiple range test ( P < 0.05).
Activity Optimized Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two/Mouse+Two+Plasmid+Activity-Optimized+CRISPR+Knockout+Library(Pooled+Library+%231000000096)/pm42557325-662-4-9
Average 93 stars, based on 1 article reviews
activity optimized sequences - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

Image Search Results


CsRNF interacts with CsHSF24 in vitro and in vivo. (A) BiFC assay demonstrating the interaction between CsHSF24 and CsRNF in N. benthamiana leaf cells. YFP fluorescence indicates successful interaction. Scale bar = 50 μm. EV = empty vector control; CsHSF24‐BC/CsRNF‐BC = background controls for CsHSF24 and CsRNF, respectively. Interaction test: Coexpression of YFPN‐CsHSF24 and YFPC‐CsRNF. (B) Yeast two‐hybrid assay confirming the interaction between CsRNF and CsHSF24. Serial dilutions (10 −1 to 10 −5 ) of yeast cultures were spotted onto selective media. (C) Schematic diagrams of the reporter and effector constructs used in dual‐luciferase assays. (D, E) Dual‐luciferase assay showing that CsRNF represses CsHSF24‐mediated activation of the CsF3′5′H2 promoter. (F) A pull‐down assay of the interaction between CsRNF and CsHSF24. Recombinant GST‐CsHSF24 was incubated with immobilized His‐CsRNF and detected for immunoblot analysis using an anti‐His antibody (upper panel) or an anti‐GST antibody (bottom panel). The arrow represents the target protein. (G) The expression level of CsRNF in leaves of one wild tea plant (“Siqiu” (“SQ”)) and three tea cultivars (“Wuniuzao” (“WNZ”), “Longjing43” (“LJ43”), “Zhongcha108” (“ZC108”)). Error bars: ±SD. Statistical significance was determined using Tukey's multiple range test ( P < 0.05).

Journal: The Plant Journal

Article Title: A novel CsRNF‐CsHSF24‐ CsF3′5′H2 module regulates epigallocatechin gallate biosynthesis in Camellia sinensis

doi: 10.1111/tpj.71155

Figure Lengend Snippet: CsRNF interacts with CsHSF24 in vitro and in vivo. (A) BiFC assay demonstrating the interaction between CsHSF24 and CsRNF in N. benthamiana leaf cells. YFP fluorescence indicates successful interaction. Scale bar = 50 μm. EV = empty vector control; CsHSF24‐BC/CsRNF‐BC = background controls for CsHSF24 and CsRNF, respectively. Interaction test: Coexpression of YFPN‐CsHSF24 and YFPC‐CsRNF. (B) Yeast two‐hybrid assay confirming the interaction between CsRNF and CsHSF24. Serial dilutions (10 −1 to 10 −5 ) of yeast cultures were spotted onto selective media. (C) Schematic diagrams of the reporter and effector constructs used in dual‐luciferase assays. (D, E) Dual‐luciferase assay showing that CsRNF represses CsHSF24‐mediated activation of the CsF3′5′H2 promoter. (F) A pull‐down assay of the interaction between CsRNF and CsHSF24. Recombinant GST‐CsHSF24 was incubated with immobilized His‐CsRNF and detected for immunoblot analysis using an anti‐His antibody (upper panel) or an anti‐GST antibody (bottom panel). The arrow represents the target protein. (G) The expression level of CsRNF in leaves of one wild tea plant (“Siqiu” (“SQ”)) and three tea cultivars (“Wuniuzao” (“WNZ”), “Longjing43” (“LJ43”), “Zhongcha108” (“ZC108”)). Error bars: ±SD. Statistical significance was determined using Tukey's multiple range test ( P < 0.05).

Article Snippet: To identify proteins that interact with CsHSF24, a Y2H screen was performed using the MATCHMAKER Gold Yeast Two‐Hybrid System (Clontech).

Techniques: In Vitro, In Vivo, Bimolecular Fluorescence Complementation Assay, Fluorescence, Plasmid Preparation, Control, Yeast, Two Hybrid Assay, Construct, Luciferase, Activation Assay, Pull Down Assay, Recombinant, Incubation, Western Blot, Analysis, Expressing